Pato

Patricio Manque

Full Professor of Center of Genomics and Bioinformatics and Center of Integrative Biology


PATRICIO.MANQUE@UMAYOR.CL


Patricio Manque obtained his Ph.D. in Microbiology and Immunology at UNIFESP-Brasil where he studied the molecular mechanisms associated with with the invasion of the protozoan parasite Trypanosoma cruzi. Afterwards, he performed a Postdoc at the same University where he developed one of the first T. cruzi transgenic parasites which made the further characterization of key pathogenic factors possible. As the genomic revolution was taking place he performed his second Postdoc at Virginia Commonwealth University, USA in parasite genomics. He participated in the full genomic sequencing of several pathogenic parasites and used many bioinformatics approaches to identify potential new vaccine candidates against the intestinal parasite Cryptosporidium. Next, he moved back to Chile where he founded the first center for genomics in Chile. In this center, he started to use systems biology approaches to study neurodegenerative diseases. He established the network biology lab where he together with Dr. Woehlbier combines systems approaches with traditional wet lab approaches to identity and characterize new genes associated with neurodegeneration.



Salvadores N, Sanhueza M, Manque P, Court FA. Axonal Degeneration during Aging and Its Functional Role in Neurodegenerative Disorders. Front Neurosci. 2017 Sep 4;11:451.

Nassif M, Woehlbier U, Manque P. The delicate balance of autophagy in neurodegeneration. Autophagy: Cancer, Other Pathologies, Inflammation, Immunity, Infection, and Aging. Academic Press. Vol. 12, 2017:387–399 (Book chapter)

Nassif M, Woehlbier U, Manque PA. The Enigmatic Role of C9ORF72 in Autophagy. Front Neurosci. 2017 Aug 3;11:442.

Sánchez C, Villacreses J, Blanc N, Espinoza L, Martinez C, Pastor G, Manque P, Undurraga SF, Polanco V. High quality RNA extraction from Maqui berry for its application in next-generation sequencing. Springerplus. 2016 Aug 3;5(1):1243.

Isaza JP, Galván AL, Polanco V, Huang B, Matveyev AV, Serrano MG, Manque P, Buck GA, Alzate JF. Revisiting the reference genomes of human pathogenic Cryptosporidium species: reannotation of C. parvum Iowa and a new C. hominis reference. Sci Rep. 2015 Nov 9;5:16324.

Villacreses J, Rojas-Herrera M, Sánchez C, Hewstone N, Undurraga SF, Alzate JF, Manque P, Maracaja-Coutinho V, Polanco V. Deep sequencing reveals the complete genome and evidence for transcriptional activity of the first virus-like sequences identified in Aristotelia chilensis (Maqui Berry). Viruses. 2015 Apr 3;7(4):1685-99.

Yañez AJ, Molina C, Haro RE, Sanchez P, Isla A, Mendoza J, Rojas-Herrera M, Trombert A, Silva AX, Cárcamo JG, Figueroa J, Polanco V, Manque P, Maracaja-Coutinho V, Olavarría VH. Draft Genome Sequence of Virulent Strain AUSTRAL-005 of Piscirickettsia salmonis, the Etiological Agent of Piscirickettsiosis. Genome Announc. 2014 Oct 16;2(5).

Rojas R, Segovia C, Trombert AN, Santander J, Manque P. The Effect of Tunicamycin on the Glucose Uptake, Growth, and Cellular Adhesion in the Protozoan Parasite Crithidia fasciculate. Curr Microbiol. 2014 Oct;69(4):541-8.

Tu E, Swenson LC, Land S, Pett S, Emery S, Marks K, Kelleher AD, Kaye S, Kaiser R, Schuelter E, Harrigan R; Manque P, MARCH Laboratory Group and the MARCH Study Group. Results of external quality assessment for proviral DNA testing of HIV tropism in the Maraviroc Switch collaborative study. J Clin Microbiol. 2013 Jul;51(7):2063-71.

Roche JK, Rojo AL, Costa LB, Smeltz R, Manque P, Woehlbier U, Bartelt L, Galen J, Buck G, Guerrant RL. Intranasal vaccination in mice with an attenuated Salmonella enterica Serovar 908htr A expressing Cp15 of Cryptosporidium: impact of malnutrition with preservation of cytokine secretion. Vaccine. 2013, Jan 30;31(6):912-8.

Manque PA*, Woehlbier U*, Lara AM, Tenjo F, Alves J and Buck GA. Identification and characterization of a novel calcium-activated apyrase from Cryptosporidium parasites and its potential role in pathogenesis. PloS One. 2012; 7(2): e31030. (*equal contribution)

Manque PA, Tenjo F, Woehlbier U, Lara AM, Serrano MG, Xu P, Alves JM, Smeltz RB, Conrad DH, Buck GA. 2011. Identification and immunological characterization of three potential vaccinogens against Cryptosporidium species. Clin Vaccine Immunol. 2011 Nov;18(11):1796-802

Manque PA, Probst CM, Pereira MC, Rampazzo RC, Ozaki LS, Pavoni DP, Silva Neto DT, Carvalho MR, Xu P, Serrano MG, Alves JM, Meirelles Mde N, Goldenberg S, Krieger MA, Buck GA. Trypanosoma cruzi infection induces a global host cell response in cardiomyocytes. Infect Immun 2011; 79(5):1855-62

Manque P, Woehlbier U. Systems Biology-Based Approaches Applied to Vaccine Development. Handbook of Research on Computational and Systems Biology: Interdisciplinary Applications, USA, IGI Global, 2011, Pages 61-78. (Book chapter)

Graça-de Souza VK, Monteiro-Góes V, Manque P, Souza TA, Corrêa PR, Buck GA, Ávila AR, Yamauchi LM, Pinge-Filho P, Goldenberg S, Krieger MA, Yamada-Ogatta SF. Sera of chagasic patients react with antigens from the tomato parasite Phytomonas serpens. Biol Res. 2010; 43(2):233-41.

Holetz FB, Alves LR, Probst CM, Dallagiovanna B, Marchini FK, Manque P, Buck G, Krieger MA, Correa A, Goldenberg S. Protein and mRNA content of TcDHH1-containing mRNPs in Trypanosoma cruzi. FEBS J. 2010. Aug;277(16):3415-26

Vanee N, Roberts SB, Fong SS, Manque P, Buck GA. 2010. A genome-scale metabolic model of Cryptosporidium hominis. Chem Biodivers. 2010. May;7(5):1026-39

Cohn B*, Manque P*, Lara AM, Serrano M, Sheth N, Buck G. 2010. Putative cis-regulatory elements associated with heat shock genes activated during excystation of Cryptosporidium parvum. PLoS One. Mar 4;5(3):e9512. (*equal contribution)

Alves LR, Avila AR, Correa A, Holetz FB, Mansur FC, Manque PA, de Menezes JP, Buck GA, Krieger MA, Goldenberg S. 2010. Proteomic analysis reveals the dynamic association of proteins with translated mRNAs in Trypanosoma cruzi. Gene. 2010 Mar 1;452(2):72-8

Cobb D, Guo S, Lara AM, Manque P, Buck G, Smeltz RB. 2009. T-bet-dependent regulation of CD8+ T-cell expansion during experimental Trypanosoma cruzi infection. Immunology. 2009 Dec;128(4):589-99

Yang YL, Serrano MG, Sheoran AS, Manque PA, Buck GA, Widmer G. 2009. Over-expression and localization of a host protein on the membrane of Cryptosporidium parvum infected epithelial cells. Mol Biochem Parasitol. Nov;168(1):95-101

Roberts SB, Robichaux JL, Chavali AK, Manque PA, Lee V, Lara AM, Papin JA, Buck GA. Proteomic and network analysis characterize stage-specific metabolism in Trypanosoma cruzi. BMC Syst Biol. 2009. May 16;3:52

Gomes IN, Palma LC, Campos GO, Lima JG, DE Almeida TF, DE Menezes JP, Ferreira CA, Santos RR, Buck GA, Manque PA, Ozaki LS, Probst CM, DE Freitas LA, Krieger MA, Veras OS. The scavenger receptor MARCO is involved in Leishmania major infection by CBA/J macrophages. Parasite Immunol. 2009. Apr;31(4):188-98

Mercado R, Buck GA, Manque PA, Ozaki LS. Cryptosporidium hominis infection of the human respiratory tract. Emerg Infect Dis. 2007. Mar;13(3):462-4

Xu P, Alves JM, Kitten T, Brown A, Chen Z, Ozaki LS, Manque P, Ge X, Serrano MG, Puiu D, Hendricks S, Wang Y, Chaplin MD, Akan D, Paik S, Peterson DL, Macrina FL, Buck GA.2007. Genome of the opportunistic pathogen Streptococcus sanguinis. J Bacteriol. 2007 Apr;189(8):3166-75

Buck GA, Mazurie A, Roberts S, Alves A, Arassapan D, Serrano M and Manque, P. Genomes to networks, pathways and function. Lecture series on computer and computacional sciencies, 2006, V 7: 758-63.

Xu P, Widmer G, Wang Y, Ozaki LS, Alves JM, Serrano MG, Puiu D, Manque P, Akiyoshi D, Mackey AJ, Pearson WR, Dear PH, Bankier AT, Peterson DL, Abrahamsen MS, Kapur V, Tzipori S, Buck GA. The genome of Cryptosporidium hominis. Nature. 2004 Oct 28; 431(7012):1107-12.

Araya JE, Neira I, da Silva S, Mortara RA, Manque P, Cordero E, Sagua H, Loyola A, Bórquez J, Morales G, González J. Diterpenoids from Azorella compacta (Umbelliferae) active on Trypanosoma cruzi. Mem Inst Oswaldo Cruz. 2003 Apr;98(3):413-8.

Manque PM, Neira I, Atayde VD, Cordero E, Ferreira AT, da Silveira JF, Ramirez M, Yoshida N. Cell adhesion and Ca2+ signaling activity in stably transfected Trypanosoma cruzi epimastigotes expressing the metacyclic stage-specific surface molecule gp82. Infect Immun. 2003 Mar;71(3):1561-5.

Yoshida N, Favoreto S Jr, Ferreira AT, Manque PM. Signal transduction induced in Trypanosoma cruzi metacyclic trypomastigotes during the invasion of mammalian cells. Braz J Med Biol Res. 2000 Mar;33(3):269-78.

Manque PM, Eichinger D, Juliano MA, Juliano L, Araya JE, Yoshida N. Characterization of the cell adhesion site of Trypanosoma cruzi metacyclic stage surface glycoprotein gp82. Infect Immun. 2000 Feb;68(2):478-84.

Ruiz RC, Favoreto S Jr, Dorta ML, Oshiro ME, Ferreira AT, Manque PM, Yoshida N. Infectivity of Trypanosoma cruzi strains is associated with differential expression of surface glycoproteins with differential Ca2+ signalling activity. Biochem J. 1998 Feb 15;330 ( Pt 1):505-11

González J, Neira I, Gutiérrez B, Anacona D, Manque P, Silva X, Marín S, Sagua H, Vergara U. Serum antibodies to Trypanosoma cruzi antigens in Atacameños patients from highland of northern Chile. Acta Trop. 1996 Feb;60(4):225-36.

González J, Ramírez C, Seguel X, Gutiérrez B, Manque P, Porcile P, Neira I, Sagua H. Levels of anti-Gal antibodies in persons infected and non-infected with Trypanosoma cruzi. Probably induced by bacteria and by the parasite. Bol Chil Parasitol. 1995 Jan-Jun;50(1-2):3-9

Patricio-linea-de-investigacion-pic1.jpg#asset:991. The lab of Network Biology
Headed jointly by Dr. Patricio Manque and myself combines systems biology with molecular biology, biochemistry, cellular and animal studies to understand mechanisms of human disease. We apply network-based approaches to build disease-specific networks allowing us to identify new genes, pathways, subnetworks responsible for the molecular pathology of a disease. To achieve this, we collect large data sets (RNAseq, CNV, HUGE, Proteomics) and combine them with a diverse set of bioinformatic tools, e.g. convergent analysis. This approach permits us, (i) to identify topological changes in the overall structure of the network, (ii) to unravel new genes or new interactions associated with a disease, and (iii) to detect potential master regulators in the disease specific networks. Once we bioinformatically identified a candidate gene we investigate its role during disease with a broad set of experimental tools. Our current focus is the characterization of novel genes inserted in the diseasosome of the neurodegenerative disease amyotrophic lateral sclerosis (ALS). In addition, by using OMICs approach our lab is focused in understanding the role of intestinal microbiome in neurodegenerative diseases.

Name: FONDECYT

Title: Defining the role of KIAA0226L/C13ORF18 in the autophagy/endolysosomal pathway during ALS pathology.

Funding Institution:CONICYT

Research in charge: Ute Woehlbier

Date of implementation: 2015-2019

Name: FONDECYT

Title: Loss of key functions drives Salmonella Typhi adaptive pathogenesis to humans.

Funding Institution: CONICYT

Research in charge: Guido Mora

Date of implementation: 2015-2019

Fernanda Hernandez Berrios
Lab Manager

Bachelor in Biotechnology Engineering from Universidad Andres Bello, Chile. In charge of general lab management and organization, in addition to molecular biology of the laboratory.
Cristian Bergmann
Ph.D. student

Bachelor in Medical Technology from Universidad Mayor, Chile. Studying the role of RUBCNL in the immunomodulatory potential of mesenchymal stem cells.
PM.jpg#asset:355Click here for zoom

National

Felipe Court, Center for Integrative Biology, Universidad Mayor, Chile

Flavio Carrion, Centro de Medicina Translacional, Universidad de Desarollo, Chile

Claudio Hetz, Biomedical Neuroscience Institute, Universidad de Chile, Chile

International

Mathieu Bertrand, Inflammation Research Center, Ghent University, Ghent, Belgium

Peter Vandenabeele, Inflammation Research Center, Ghent University, Ghent, Belgium

Gregory Buck, Center for Biological Complexity, Virginia Commonwealth University, Richmond, USA