Located in the CIB (Central Biomedical Research Center), on the Huerchuraba campus of the Faculty of Sciences, UM2 has a dedicated team of imaging scientists with extensive experience in optical microscopy. We support our users at every stage of their research, from formulating the scientific question and experimental design to collecting, analyzing, and presenting results. We have advanced confocal and wide-field microscopes and operate the only light-sheet system in Chile. We also have a dedicated image analysis team running IMARIS, MATLAB, Fiji, and other open-source analysis programs.
We offer support in experimental design, equipment selection, training, image processing, data analysis, and presentation of results.
The LS7 microscope is part of the LiSIUM (Light-Sheet Imaging at Universidad Mayor) project. Light-sheet fluorescence microscopy enables rapid imaging with low phototoxicity and fluorophore bleaching thanks to its single-plane illumination, which illuminates samples orthogonally with dedicated illumination objectives and low excitation laser power. The LS7 enables cellular resolution imaging of live or fixed, transparent or clarified specimens up to 2 cm in size and samples undergoing expansion protocols.
The Leica SP8 microscope is an advanced confocal microscopy system, equipped with a spectral detection system that enables acquisition at multiple wavelengths. It enables advanced microscopy techniques such as FRET and FRAP.
The Leica DMi8 microscope is a wide-field inverted microscope with a highly sensitive camera and an efficient light management system, producing bright, clear images while minimizing phototoxicity. It is equipped with a motorized stage that allows for the generation of a complete map of the slide or plate containing the sample.
